In the hematopoietic system, tyrosine kinases of Syk family are essential components of immunoreceptor ITAM-based signaling. While an increasing number of data involved immunoreceptors in neural functions, the contribution of Syk kinases remains obscure. In previous studies we depicted phosphorylated forms of Syk kinases in specialized populations of migrating neurons or projecting axons. Moreover, we identified ephrin/Eph as guidance molecules utilizing the ITAM-bearing molecule CD3zeta and associated Syk kinases for growth cone collapsing response induced in vitro. From here, we show that in the developing spinal cord, Syk is phosphorylated in navigating commissural axons. By analyzing axon trajectories in open book preparations of Syk−/− ; ZAP-70−/− double KO embryos, we found that Syk kinases are dispensable for attraction towards the midline but confer growth cone responsiveness to repulsive signals required to expel commissural axons from the midline. Known to serve repulsive function at midline, ephrinB3/EphB2 consist in obvious candidates in driving the Syk-dependent repulsive response. Indeed, Syk kinases were found as required for ephrinB3-induced growth cone collapse in cultured commissural neurons. Besides, in fragments of commissural neuron-enriched tissues, Syk is present under a constitutively phosphorylated state and ephrinB3 decreases its level of phosphorylation. Furthermore, directly altering Syk kinase activity through pharmacological inhibition was sufficient to induce growth cone collapse, suggesting that Syk inhibition is a general requirement for growth cone collapse. In conclusion, Syk kinases act as a molecular switch of growth cone adhesive and repulsive responses.