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Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using click chemistry.

Journal article published in 2007 by Ravindra Kumar, Marcus Wilhelmsson, Afaf El-Sagheer, John Tumpane, Per Lincoln, Lm Marcus Wilhelmsson, Tom Brown ORCID
This paper was not found in any repository; the policy of its publisher is unknown or unclear.
This paper was not found in any repository; the policy of its publisher is unknown or unclear.

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Abstract

The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5'-alkyne and the other with a 3'-azide, to produce a DNA strand with an unnatural backbone at the ligation point. A template-free click-ligation reaction has been used for the intramolecular circularization of a single stranded oligonucleotide which was used as a template for the synthesis of a covalently closed DNA catenane.