Dissemin is shutting down on January 1st, 2025

Published in

Nature Research, Nature Methods, 9(9), p. 913-915, 2012

DOI: 10.1038/nmeth.2137

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Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules

Journal article published in 2012 by Jerrod J. Schwartz, Choli Lee, Jay Shendure ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We present dial-out PCR, a highly parallel method for retrieving accurate DNA molecules for gene synthesis. A complex library of DNA molecules is modified with unique flanking tags before massively parallel sequencing. Tag-directed primers then enable the retrieval of molecules with desired sequences by PCR. Dial-out PCR enables multiplex in vitro clone screening and is a compelling alternative to in vivo cloning and Sanger sequencing for accurate gene synthesis.