Published in
Colégio Brasileiro de Patologia Animal - CBPA, Pesquisa Veterinária Brasileira, 4(35), p. 353-359
DOI: 10.1590/s0100-736x2015000400007
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Effect of Lactobacillus sp. isolates supernatant on Escherichia coli O157:H7 enhances the role of organic acids production as a factor for pathogen control
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Rivaldi,Javier D.,
Thais S. Coutinho,
Claudete S. Astolfi Ferreira,
Ferreira,Antonio J. Piantino,
Antonio J. Piantino Ferreira ,
Antonio J. Piantino Ferreira,
Mancilha,Ismael M.,
Ismael M. Mancilha
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Abstract
Many attempts have been made to establish the control of foodborne pathogens through Lactobacillus isolates and their metabolism products with success being obtained in several situations. The aim of this study was to investigate the antagonistic effect of eight Lactobacillus isolates, including L. casei subsp. pseudoplantarum, L. plantarum, L. reuteri and L. delbrueckii subsp. delbrueckii, on the pathogenic Escherichia coli strain O157:H7. The inhibitory effect of pure cultures and two pooled cultures supernatants of Lactobacillus on the growth of pathogenic bacteria was evaluated by the spot agar method and by monitoring turbidity. Antimicrobial activity was confirmed for L. reuteri and L. delbrueckii subsp. delbrueckii and for a pool of lactic acid bacteria. The neutralized supernatant of the pool exerted a higher antimicrobial activity than that of the individual strains. Furthermore, D-lactic acid and acetic acid were produced during growth of the Lactobacillus isolates studied.