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Optica, Biomedical Optics Express, 4(6), p. 1353, 2015

DOI: 10.1364/boe.6.001353

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A new strategy for label-free detection of lymphoma cancer cells

Journal article published in 2015 by Nicola M. Martucci, Ilaria Rea, Immacolata Ruggiero, Monica Terracciano, Luca De Stefano ORCID, Nunzia Migliaccio, Camillo Palmieri, Giuseppe Scala, Paolo Arcari, Ivo Rendina, Annalisa Lamberti
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

In this paper, a new strategy for highly selective and sensitive direct detection of lymphoma cells by exploiting the interaction between a peptide and its B-cell receptor, has been evaluated. In particular, an idiotype peptide, able to specifically bind the B-cell receptor of A20 cells in mice engrafted with A20 lymphoma, has been used as molecular probe. The new detection technique has been demonstrated on a planar crystalline silicon chip. Coverage of 85% of silicon surface and detection efficiency of 8.5 × 10−3 cells/μm2 were obtained. The recognition strategy promises to extend its application in studying the interaction between ligands and their cell-surface receptors.