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Springer, Analytical and Bioanalytical Chemistry, 3(390), p. 833-840, 2007

DOI: 10.1007/s00216-007-1564-9

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Characterization of cellular chemical dynamics using combined microfluidic and Raman techniques

Journal article published in 2007 by Xunli Zhang, Huabing Yin ORCID, Jon M. Cooper, Stephen J. Haswell
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The integration of a range of technologies including microfluidics, surface-enhanced Raman scattering and confocal microspectroscopy has been successfully used to characterize in situ single living CHO (Chinese hamster ovary) cells with a high degree of spatial (in three dimensions) and temporal (1 s per spectrum) resolution. Following the introduction of a continuous flow of ionomycin, the real time spectral response from the cell was monitored during the agonist-evoked Ca2+ flux process. The methodology described has the potential to be used for the study of the cellular dynamics of a range of signalling processes.