Wiley, FEBS Letters, 1-3(565), p. 111-116, 2004
DOI: 10.1016/j.febslet.2004.03.086
Wiley, FEBS Letters, 1-3(565), p. 111-116
DOI: 10.1016/s0014-5793(04)00392-8
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The open reading frame 3 of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes a predicted protein 3a, consisting of 274 amino acids, that lacks any significant similarities to any known protein. We generated specific antibodies against SARS protein 3a by using a synthetic peptide (P2) corresponding to amino acids 261-274 of the putative protein. Anti-P2 antibodies and the sera from SARS patients could specifically detect the recombinant SARS protein 3a expressed in Escherichia coli and in Vero E 6 cells. Expression of SARS protein 3a was detected at 8-12 h after infection and reached a higher level after similar to24 h in SARS-CoV-infected Vero E6 cells. Protein 3a was also detected in the alveolar lining pneumocytes and some intra-alveolar cells of a SARS-CoV-infected patient's lung specimen. Recombinant protein 3a expressed in Vero E6 cells and protein 3a in the SARS-CoV-infected cells was distributed over the cytoplasm in a fine punctate pattern with partly concentrated staining in the Golgi apparatus. Our study demonstrates that SARS-CoV indeed expresses a novel protein 3a, which is present only in SARS-CoV and not in other known CoVs. ; 附設醫院醫學研究部 ; 醫學院附設醫院 ; 期刊論文