We describe opinions on medical use of Cannabis sativa L. under conditions of Slovakia ( n = 717). Personal experience with marijuana was detected in 77.42% ( n = 553) in age categories younger than 20 years ( n = 96) and in 77.06% ( n = 457) of adults. Almost 86% of respondents ( n = 618) agreed with legal use of marijuana for medical purposes. Furthermore, respondents’ views on legal usage of marijuana for medical purposes could be affected by availability of information ( r = .12) and personal experience ( r = .23). Negative impact of substance abuse in younger age-groups was recognized as threat ( r = .47), but knowledge about harmful effect of marijuana use did not affect personal opinion for decriminalization ( r = .38).