Dissemin is shutting down on January 1st, 2025

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Taylor and Francis Group, Epigenetics, 6(9), p. 829-833

DOI: 10.4161/epi.28790

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Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray

Journal article published in 2014 by Sebastián Moran ORCID, Miguel Vizoso ORCID, Anna Martinez-Cardús, Antonio Gomez ORCID, Xavier Matías-Guiu, Sebastián M. Chiavenna, Andrés G. Fernandez, Manel Esteller
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

A formalin-fixed paraffin-embedded (FFPE) sample usually yields highly degraded DNA, which limits the use of techniques requiring high-quality DNA, such as Infinium Methylation microarrays. To overcome this restriction, we have applied an FFPE restoration procedure consisting of DNA repair and ligation processes in a set of paired fresh-frozen (FF) and FFPE samples. We validated the FFPE results in comparison with matched FF samples, enabling us to use FFPE samples on the Infinium HumanMethylation450 Methylation array.