Prion Strain Discrimination Based on Rapid In Vivo Amplification and Analysis by the Cell Panel Assay

Karapetyan, Yervand Eduard; Saá, Paula; Mahal, Sukhvir Paul; Sferrazza, Gian Franco; Sherman, Alexandra; Salès, Nicole; Weissmann, Charles; Lasmézas, Corinne Ida

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Public Library of Science, PLoS ONE, 5(4), p. e5730, 2009

DOI: 10.1371/journal.pone.0005730

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Prion Strain Discrimination Based on Rapid In Vivo Amplification and Analysis by the Cell Panel Assay

Journal article published in 2009 by Yervand Eduard Karapetyan, Paula Saá

, Sukhvir Paul Mahal, Gian Franco Sferrazza, Alexandra Sherman, Nicole Salès, Charles Weissmann, Corinne Ida Lasmézas

This paper is made freely available by the publisher.

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Abstract

Prion strain identification has been hitherto achieved using time-consuming incubation time determinations in one or more mouse lines and elaborate neuropathological assessment. In the present work, we make a detailed study of the properties of PrP-overproducing Tga20 mice. We show that in these mice the four prion strains examined are rapidly and faithfully amplified and can subsequently be discriminated by a cell-based procedure, the Cell Panel Assay.

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Prion Strain Discrimination Based on Rapid In Vivo Amplification and Analysis by the Cell Panel Assay

Abstract