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Nature Research, Nature Methods, 12(9), p. 1202-1205, 2012

DOI: 10.1038/nmeth.2249

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Two-photon optogenetics of dendritic spines and neural circuits.

Journal article published in 2012 by Am Packer ORCID, Ds Peterka, Jan Jj Hirtz, Rohit Prakash, Karl Deisseroth, Rafael Yuste
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We demonstrate a two-photon optogenetic method that generates action potentials in neurons with single-cell precision, using the red-shifted opsin C1V1(T). We applied the method to optically map synaptic circuits in mouse neocortical brain slices and to activate small dendritic regions and individual spines. Using a spatial light modulator, we split the laser beam onto several neurons and performed simultaneous optogenetic activation of selected neurons in three dimensions.