Fig. 1. A) Sheets of large round to polygonal pleomorphic cells with atypical nuclei, conspicuous nucleoli and abundant eosinophilic cytoplasm; numerous apoptotic bodies are evident, predominantly in the upper field (Hematoxylin & Eosin, original magnification × 200). B) Nuclear staining of SMARCA4 is lost in the tumor cells. C) Nuclear staining for SMARCB1 is retained. D) Wildtype (top) and mutated (bottom) chromatograms of the germline (left, c.1641_1641delC; p.D547Efs*66) and somatic (right, c.1714A T; p.K572*) mutations. The chromatogram of the somatic mutation was taken from the sequence of the reverse complement, as the nucleotide change was near the position of the germline deletion, and therefore not as clear in the forward trace.