Published in

Optica, Biomedical Optics Express, 8(6), p. 2778, 2015

DOI: 10.1364/boe.6.002778

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Macro-optical trapping for sample confinement in light sheet microscopy

Journal article published in 2015 by Zhengyi Yang, Peeter Piksarv, David E. K. Ferrier ORCID, Frank J. Gunn-Moore, Kishan Dholakia
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Light sheet microscopy is a powerful approach to construct three-dimensional images of large specimens with minimal photo-damage and photo-bleaching. To date, the specimens are usually mounted in agents such as agarose, potentially restricting the development of live samples, and also highly mobile specimens need to be anaesthetized before imaging. To overcome these problems, here we demonstrate an integrated light sheet microscope which solely uses optical forces to trap and hold the sample using a counter-propagating laser beam geometry. Specifically, tobacco plant cells and living Spirobranchus lamarcki larvae were successfully trapped and sectional images acquired. This novel approach has the potential to significantly expand the range of applications for light sheet imaging.