A molecular phylogeographic study using a fragment of the mitochondrial gene for cytochrome b (cytb) was performed on the lesser white-toothed shrew, Crocidura suaveolens, from seven localities in central and southern Italy. Comparison with cytb European haplotypes revealed the absence of endemic lineages in the region, in contrast to what has been observed for many other Italian terrestrial vertebrates. Indeed the all the Italian specimens results nested with Balkanic conspecific within an Italo-Balkan clade. Historical demography of this clade showed a scenario of expansion which preceded the LGM. This evidence of glacial persistence indicates a certain flexibility of the classic models of Pleistocene biogeography.