薄膜（membrane）處理在近十餘年已成為國際間各種工業或都市污水處理技術最主要研究議題之一。本研究為結合傳統循序批分式生物反應槽（sequencing batch bioreactor, SBR）及薄膜生物處理程序（membrane bioreactor, MBR）而發展出循序批分式薄膜生物反應槽（sequencing batch membrane bioreactor, SBMBR），以間歇曝氣方式提高含碳、氮、磷成分廢水之處理效率，並以SBMBR模廠所馴養出的污泥，利用批次實驗探討脫硝除磷現象，且由於經本SBMBR模廠所馴養之污泥特性為NO2-N累積，此與一般文獻大多為NO3-N累積不同，故本研究之重點為探討NO3-N及NO2-N之脫硝除磷現象，以瞭解不同電子接受者在攝磷反應中所扮演的角色。由薄膜特性實驗結果，可知薄膜操作之最佳條件為泵浦轉速30 rpm，每天固定時間利用清水、同轉速反沖洗5分鐘，當TMP達20 kPa時，以5,000 mg/L NaOCl＋2％ NaOH之化學藥劑浸洗薄膜15小時。以此操作條件進行SBMBR實驗，結果兩片MF薄膜分別可連續操作91及100天後，才需進行藥洗步驟。由日常監測結果可知SBR模廠之SS、COD、TN及TP去除率分別為90.4、93.8、69.2及85.3％；而SBMBR模廠之SS、COD、TN及TP去除率分別為96.7、95.52、73.6及98.1％。由此可知SBMBR之處理效率優於SBR。且SBMBR程序對於有機物及磷去除效率極佳，但除氮效果則與SBR程序差異不大，此由 SBR及SBMBR出流水之TKN分別為22.9及0 mg-N/L，但NOX-N分別為4.2及21.2 mg-N/L，顯示SBMBR模廠雖有極佳之銨氧化效果，但脫硝效率卻甚差，因此導致兩個模廠之TN去除率相近。由本研究之脫硝攝磷批次實驗結果，可知本SBMBR模廠存在有DNPAO及non-DNPAO，且PO、PNO3及PNO2皆可進行攝磷反應，以NO3-N與NO2-N之混合為電子接受者時，PNO3 + NO2亦可進行攝磷反應。但NO3-N及NO2-N濃度過高時會抑制攝磷行為，唯無法精準得知當其濃度高於某值時，將會開始抑制攝磷反應；且NO2-N濃度低於5 mg-N/L時，並不會抑制攝磷反應。由於本模廠為NO2-N累積，導致PNO2為本模廠DNPAO之優勢菌種，因此攝磷率較PNO3高，且本模廠存在有只能單獨使用PNO3或PNO2攝磷之DNPAO。而由PNO3 + NO2之攝磷率（39.0 mg-P/g MLVSS-hr）低於PNO3與PNO2（27.84及37.44 mg-P/g MLVSS-hr）之和，可知本模廠存在有一DNPAO可同時利用PNO3及PNO2進行攝磷反應。由此可知，本模廠之DNPAO至少可分為三類，其一可單獨利用PNO3進行攝磷行為；另一可單獨利用PNO2進行攝磷行為；此外尚有可同時利用PNO3及PNO2進行攝磷行為之DNPAO。 ; Membrane is one of the main studies to treat various kinds of industrial and domestic wastewaters in nearly decades. This research combines two kinds of system such as SBR (sequencing batch bioreactor) and MBR (membrane bioreactor) to establish an SBMBR (sequencing batch membrane bioreactor), and using intermittent aeration to enhance the removal efficiency of organics and nutrients of wastewaters. Results from a series of batch experiments were presented, with the sludge obtained from SBMBR, revealed that the sludge characteristic is accumulated for nitrite, mostly different from other investigations (for nitrate accumulated). The purpose of this research is to validate and study the role of different electron acceptors in phosphate-uptake process. Experimental results showed that the optimum conditions are rotational speed 30 rpm, five minutes of backwash with branchwater and the same rotational speed daily, and chemical washing with 5,000 mg/L + 2％ NaOH when the TMP up to 40 kPa. Under this control, two microfiltration systems could be operated for 91 and 100 days respectively without any cleaning procedure. Monitor result revealed that the removal efficiency of SS, COD, TN and TP is 90.4%, 93.8%, 69.2% and 85.3% respectively in SBR; 96.7%, 95.52%, 73.6% and 98.1% respectively in SBMBR. Therefore the treatment efficiency of SBMBR was superior to SBR. Although results showed that high removal efficiency of organics and phosphorus in SBMBR, nitrogen removal was similar to SBR. The concentrations of effluent TKN were 22.9 and 0 mg-N/L respectively, but NOx-N were 4.2 and 21.2 mg-N/L respectively in SBR and SBMBR. The experimental results showed that the ammonium oxidization of SBMBR was satisfying, but the denitrification effect was unsatisfying, so TN removal of SBMBR was closed to SBR. Results from denitrification/phosphate-uptake batch experiments revealed the existence of DNPAO and non-DNPAO in SBMBR. Three types of possible electron acceptors (oxygen, nitrate and nitrite) were examined for their roles in phosphate uptake, and mixtures of nitrate and nitrite (PNO3+NO2) could phosphate uptake as well. The inhibiting effect of nitrate and nitrite was found at high concentrations, but it was unable to realize the critical concentration perfectly. The experimental results showed that nitrite at low concentration(less than 5 mg-N/L) was not detrimental to anoxic phosphate uptake. The results also suggested that SBMBR was accumulated for nitrite and led to PNO2 was the advantaged DNPAO, so the phosphate uptake rate was higher than PNO3. The results obtained indicated the existence of DNPAO that could only use nitrate or nitrite as electron acceptor, and there also existed one DNPAO could utilize nitrate and nitrite as electron acceptor simultaneously in SBMBR. Therefore the DNPAO of SBMBR could be divided to three species at least, PNO3 can utilize nitrate to uptake phosphate; PNO2 can utilize nitrite to uptake phosphate; and PNO3+NO2 can utilize nitrate and nitrite to uptake phosphate simultaneously.