Dissemin is shutting down on January 1st, 2025

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Wiley, Muscle & Nerve, 2(42), p. 268-270, 2010

DOI: 10.1002/mus.21700

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Sequencing protocols to genotype mdx, mdx4cv and mdx5cv mice

Journal article published in 2010 by Glen B. Banks, Ariana C. Combs, Jeffrey S. Chamberlain ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Currently available polymerase chain reaction (PCR) genotyping methods for point mutations in the mouse dystrophin gene can lead to false positives and result in wasted time and money due to breeding or treating the wrong mice. Here we describe a simple and accurate method for sequencing the point mutations in mdx, mdx(4cv), and mdx(5cv) mice. This method clearly distinguishes between wildtype, heterozygous, and mutant transcripts, and thereby time and money can be saved by avoiding false positives.