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Nature Research, Scientific Data, 1(4), 2017

DOI: 10.1038/sdata.2017.93

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Viral to metazoan marine plankton nucleotide sequences from the Tara Oceans expedition

Journal article published in 2017 by Jean-Marc Aury ORCID, Adriana Alberti ORCID, Shahinaz Gas, Julie Guy, Julie Poulain, Maud Haquelle, E'krame Jacoby, Olivier Jaillon ORCID, Stefan Engelen, Arnaud Lemainque, Eric Pelletier, Karine Labadie, Gaëlle Samson, Mark Wessner, Pascal Bazire and other authors.
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Data provided by SHERPA/RoMEO

Abstract

AbstractA unique collection of oceanic samples was gathered by the Tara Oceans expeditions (2009–2013), targeting plankton organisms ranging from viruses to metazoans, and providing rich environmental context measurements. Thanks to recent advances in the field of genomics, extensive sequencing has been performed for a deep genomic analysis of this huge collection of samples. A strategy based on different approaches, such as metabarcoding, metagenomics, single-cell genomics and metatranscriptomics, has been chosen for analysis of size-fractionated plankton communities. Here, we provide detailed procedures applied for genomic data generation, from nucleic acids extraction to sequence production, and we describe registries of genomics datasets available at the European Nucleotide Archive (ENA, www.ebi.ac.uk/ena). The association of these metadata to the experimental procedures applied for their generation will help the scientific community to access these data and facilitate their analysis. This paper complements other efforts to provide a full description of experiments and open science resources generated from the Tara Oceans project, further extending their value for the study of the world’s planktonic ecosystems.