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Bentham Science Publishers, Protein and Peptide Letters, 1(6), p. 7-14, 1999

DOI: 10.2174/092986650601221107152340

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Cytokine rhGM-CSF interaction with peptide fragment of hGM-CSF receptor α-chain monitored by NILIA-CD spectroscopy

Journal article published in 1999 by Rohanah Hussain, Paolo Rovero ORCID, Giuliano Siligardi, Claudia Galoppini
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Pl 15 a peptide fragment of hGM-CSFR a-chain was observed to interact selectively to cytokine rhGM-CSF (Kd=35µM) and not to IL-3 in solution by non-immobilised ligand interaction assay (NILIA) by CD spectroscopy in the spectral region characteristic ofTrp aromatic side-chain and disulphide bond chromophores (290-330mn). The information provided by NILIA-CD identified two continuous peptide fragments of GM­ CSF that could be used as a good candidate to bind to GM-CSF receptor a-chain. We propose this approach of combining spectroscopic and crystallographic information to guide the search for receptor ligand.