Published in

Future Medicine, BioTechniques, 3(29), p. 500-506, 2000

DOI: 10.2144/00293st01

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SNP genotyping by combination of 192-well MADGE, ARMS and computerized gel image analysis

Journal article published in 2000 by Sd O'Dell, Gaunt Tr, Tom R. Gaunt ORCID, Inm Day
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

Full text: Unavailable

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Postprint: archiving allowed
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Published version: archiving allowed
Data provided by SHERPA/RoMEO

Abstract

A new modification of the microplate array diagonal gel electrophoresis (MADGE) system accommodates the dual amplification refractory mutation system (ARMSTM) products of 96 samples on one 192-well gel. Simultaneous electrophoresis of a number of horizontal ARMS-MADGE gels achieves high throughput. Gels are imaged digitally, here using the FluorImager® 595 fluorescent scanning system. Customised software by PhoretixTM enables rapid computerized calling of band patterns in ARMS-MADGE arrays, in which the two wells receiving a pair of allele-specific assays for a single template are juxtaposed to form one virtual track, with genotype data exported directly into Microsoft® Excel® for statistical analysis. An ARMS assay of the A/T base change at the −23/HphI RFLP in the insulin gene promoter, which initiates from 2.5 ng template DNA, was used here to demonstrate this improved general approach for population SNP analyses.