Additional file 6: Figure S5. Triacylglycerol quantification after induced transcriptional regulation for both activator and repressor. TAG production was engineered in three yeast strains; single gRNAs targeting position TSS-139 on pDGA1 (Sc-164 and Sc-165; pERA-122, pERA-127) and TSS-381 on pOLE1 (Sc-162 and Sc-163: pERA-112, pERA-117) and multiplexed expression of both gRNAs coupled to dCas9-VPR (Sc-166 and Sc-167: pERA-132, pERA-133). The strains were cultivated in shake flasks for twenty-four hours at 200 rpm, 30 °C. Results are based on three biological replicates. Significant changes are indicated by asterisk.