Published in

Elsevier, New Biotechnology, (33), p. S66, 2016

DOI: 10.1016/j.nbt.2016.06.952

Links

Tools

Export citation

Search in Google Scholar

Engineering yeast metabolism for production of fuels and chemicals

Journal article published in 2016 by Jens B. Nielsen
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

Full text: Unavailable

Green circle
Preprint: archiving allowed
Green circle
Postprint: archiving allowed
Green circle
Published version: archiving allowed
Data provided by SHERPA/RoMEO

Abstract

Metabolic engineering relies on the Design-Build-Test cycle. This cycle includes technologies like mathematical modeling of metabolism, genome editing and advanced tools for phenotypic characterization. In recent years there have been advances in several of these technologies, which has enabled faster development of metabolically engineered strains that can be used for production of fuels and chemicals. The yeast Saccharomyces cerevisiae is widely used for production of fuels, chemicals, pharmaceuticals and materials. Through metabolic engineering of this yeast a number of novel industrial processes have been developed over the last 10 years. Besides its wide industrial use, S. cerevisiae also serves as an eukaryal model organism, and many systems biology tools have therefore been developed for this organism. These tools can be used for detailed phenotypic characterization as well as for metabolic design. In this lecture it will be demonstrated how the Design-Build-Test cycle of metabolic engineering has allowed for development of yeast cell factories for production of a range of different fuels and chemicals. Some examples of different technologies will be presented together with examples of metabolic engineering designs, in particular for development of platform strains that can be used for production of a fatty acid derived products, e.g. fatty alcohols and alkanes. It will be argued that with advancement in genome-editing technologies and novel methods for rapid phenotypic screening, advancement in the field is hampered by our design abilities, i.e. to predict genotype–phenotype connections. For this genome-scale metabolic modeling is a strong technology, and in the presentation recent advancements in mathematical modeling for cell factory design will be presented. Finally, the presentation will also demonstrate how the Design-Build-Test cycle can be expanded to incorporate adaptive laboratory evolution to identify targets for engineering complex traits, such as improved tolerance to toxic metabolites like elevated temperatures or low pH.