Additional file 5: Figure S3. of Long intergenic non-coding RNA 00152 promotes lung adenocarcinoma proliferation via interacting with EZH2 and repressing IL24 expression
Published in 2017 by
Qin-Nan Chen,
Xin Chen,
Zhen-Yao Chen,
Feng-Qi Nie,
Chen-Chen Wei,
Hong-Wei,
Li Wan,
Shuai Yan,
Sheng-Nan Ren,
Zhao-Xia Wang
Abstract
LINC00152 could not recruit LSD1 to IL24 promoter. (a) LSD1 expression in A549 and SPCA1 cells transfected with si-LSD1. (b,c) qRT-PCR and western blotting were performed to detect the IL24 mRNA and protein level in A549 and SPCA1 cells. (d) ChIP-qRT-PCR of LSD1 occupancy and H3K4me2 binding in the IL24 promoter in A549 and SPCA1 cells treated with si-LINC00152(48h) or scrambled siRNA, IgG was used as a negative control. Values are shown as the mean Âą s.d in three independent experiments. *P