AST3-1 is the second-generation wide-field optical photometric telescope dedicated to time domain astronomy at Dome A, Antarctica. Here we present the results of $i$ band images survey from AST3-1 towards one Galactic disk field. Based on time-series photometry of 92,583 stars, 560 variable stars were detected with $i$ magnitude $≤$ 16.5 mag during eight days of observations; 339 of these are previously unknown variables. We tentatively classify the 560 variables as 285 eclipsing binaries (EW, EB, EA), 27 pulsating variable stars ($δ$~Scuti, $γ$~Doradus, $δ$~Cephei variable and RR Lyrae stars) and 248 other types of variables (unclassified periodic, multi-periodic and aperiodic variable stars). Among the eclipsing binaries, 34 show O'Connell effects. One of the aperiodic variables shows a plateau light curve and another one shows a secondary maximum after peak brightness. We also detected a complex binary system with RS CVn-like light curve morphology; this object is being followed-up spectroscopically using the Gemini South telescope. ; Comment: Accepted for publication in The Astronomical Journal