Single-photon stimulated four wave mixing (StFWM) processes have great potential for photonic quantum information processing, compatible with optical communication technologies and integrated optoelectronics. In this paper, we demonstrate single-photon StFWM process in a piece of optical fiber, with seeded photons generated by spontaneous four wave mixing process (SpFWM). The effect of the single-photon StFWM is confirmed by time-resolved four-photon coincidence measurement and variation of four-photon coincidence counts under different seed-pump delays. According to the experiment results, the potential performance of quantum cloning machine based on the process is analyzed.