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American Society for Microbiology, Applied and Environmental Microbiology, 17(74), p. 5541-5548, 2008

DOI: 10.1128/aem.00342-08

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Distinct Contributions of the Nisin Biosynthesis Enzymes NisB and NisC and Transporter NisT to Prenisin Production by Lactococcus lactis

Journal article published in 2008 by H. Bart van den Berg van Saparoea ORCID, Patrick J. Bakkes, H. Bart van den Berg van Saparoea, Gert N. Moll, Arnold J. M. Driessen
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT Several Lactococcus lactis strains produce the lantibiotic nisin. The dedicated enzymes NisB and NisC and the transporter NisT modify and secrete the ribosomally synthesized nisin precursor peptide. NisB can function in the absence of the cyclase NisC, yielding the dehydrated prenisin that lacks the thioether rings. A kinetic analysis of nisin production by L. lactis NZ9700 demonstrated that the prenisin was released from the cell into the medium before the processing of the leader sequence occurred. Upon the deletion of nisC , the production of prenisin was reduced by 70%, while in the absence of nisB , the production of prenisin was nearly completely abolished. In cells lacking nisT , no secretion was observed, while the expression of nisABC in these cells resulted in considerable growth rate inhibition caused by the intracellular accumulation of active nisin. Overall, these data indicate that the efficiency of prenisin transport by NisT is markedly enhanced by NisB, suggesting a channeling mechanism of prenisin transfer between the nisin modification enzymes and the transporter.