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American Association for the Advancement of Science, Science, 6149(341), p. 1009-1012, 2013

DOI: 10.1126/science.1240985

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Molecular Basis of Tubulin Transport Within the Cilium by IFT74 and IFT81

Journal article published in 2013 by Sagar Bhogaraju ORCID, Lukas Cajanek, Cécile Fort, Thierry Blisnick, Kristina Weber, Michael Taschner, Naoko Mizuno, Stefan Lamla, Philippe Bastin, Erich A. Nigg ORCID, Esben Lorentzen
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue experiments with point mutants showed that tubulin binding by IFT81 was required for ciliogenesis in human cells.