Published in
Public Library of Science, PLoS ONE, 7(10), p. e0132455, 2015
DOI: 10.1371/journal.pone.0132455
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- ORCID
- Public Library of Science
- [www.ncbi.nlm.nih.gov] | PDF
- [hdl.handle.net] | PDF
- [research-information.bristol.ac.uk] | PDF
- [research-information.bristol.ac.uk] | PDF
- [europepmc.org] | PDF
- [www.researchgate.net] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
- [research-information.bris.ac.uk] | PDF
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Proteasome Activity Is Affected by Fluctuations in Insulin-Degrading Enzyme Distribution
,
Manuela Bozzi,
Magda Gioia,
Chiara Ciaccio,
Umberto Tarantino,
Andrea Brancaccio,
Massimo Coletta ,
Stefano Marini
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Abstract
Insulin-Degrading-Enzyme (IDE) is a Zn 2+ -dependent peptidase highly conserved throughout evolution and ubiquitously distributed in mammalian tissues wherein it displays a prevalent cytosolic localization. We have recently demonstrated a novel Heat Shock Protein-like behaviour of IDE and its association with the 26S proteasome. In the present study, we examine the mechanistic and molecular features of IDE-26S proteasome interaction in a cell experimental model, extending the investigation also to the effect of IDE on the enzymatic activities of the 26S proteasome. Further, kinetic investigations indicate that the 26S proteasome activity undergoes a functional modulation by IDE through an extra-catalytic mechanism. The IDE-26S proteasome interaction was analyzed during the Heat Shock Response and we report novel findings on IDE intracellular distribution that might be of critical relevance for cell metabolism.