Published in
American Society for Microbiology, Antimicrobial Agents and Chemotherapy, 1(51), p. 350-353, 2007
DOI: 10.1128/aac.00985-06
Links
- ORCID
- American Society for Microbiology | PDF
- [www.ncbi.nlm.nih.gov] | PDF
- [europepmc.org] | PDF
- [www.researchgate.net] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
Tools
Simple Colorimetric Inhibition Assay of Heme Crystallization for High-Throughput Screening of Antimalarial Compounds
,
Dinh Thanh Uyen,
Atsushi Maeda,
Dai Thi Xuan Trang,
Tatsuo Oida,
Shigeharu Harada,
Kaeko Kamei
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Abstract
ABSTRACT Current assays for screening new antimalarials need initiators of β-hematin formation that require laborious preparation, special devices, and substrates. In this study, based on reduction of heme absorption in β-hematin formation, we developed a simple colorimetric assay using Tween 20 as an initiator and a microplate reader for high-throughput screening of inhibitors of β-hematin formation.