Analysis of the differentiation potential of HPC/HSC-iPSCs into hematopoietic cells by flow cytometry. In the first column, cells were isolated based on size, as indicated by SSC and FSC (forward scatter). In the second column, GFP-negative cells were further selected. In the third column, c-Kit and Sca-1 double-positive (c-Kit+Sca-1+) cells were isolated. In the fourth column, c-Kit+Sca-1+ cells were further divided into CD34+ and CD34- subpopulations. APC adipose progenitor cell, MEF mouse embryonic fibroblast, HPC/HSC hematopoietic progenitor and stem cell, iPSC induced pluripotent stem cell, SSC side scatter, FSC forward scatter, APC allophycocyanin, PE-Cy7 phycoerythrin-Cy7, CD cluster of differentiation. (TIF 3169Â kb)