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Additional file 6: of Medium throughput bisulfite sequencing for accurate detection of 5-methylcytosine and 5-hydroxymethylcytosine

This paper is available in a repository.
This paper is available in a repository.

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Abstract

Using this customized BS-Seq method requires users to modify the sequencing reagent cartridge provided by Illumina. These are the operational steps for performing customized MiSeq sequencing run with customized sequencing primers (CS1, CS2, and CS2rc). Arrows indicate the transferring of fluid from fro one tube number to another on Illumina’s reagent cartridge. (1) The contents of tube 12 should be placed in tube 18. (2) The contents of tube 13 should be placed in tube 19. (3) The contents of tube 14 should be placed in tube 20. (PPTX 1142 kb)