Additional file 6: Figure S4. SVA elements capture BORIS binding sites from unique gene promoters. (A) BORIS ChIP-seq and deep CAGE (Cap Analysis of Gene Expression)-seq (ENCODE data) coverage tracks for the MAST2 gene (upper track) and for the SVA-F1 element (lower track) in K562 cells. BORIS occupancy at the MAST2 first exon sequence coincided with the multiple transcription start sites (TSS) for MAST2 and SVA-F1 family expression in K562 cells. The black arrows show the direction of transcription based on CAGEs enrichment on plus strand. The red double-headed arrows show the MAST2 sequence captured by SVA-F1 family from the MAST2 gene. (B) ChIP-seq enrichment of BORIS occupancy depends on the number of BORIS binding sites in the transduced sequences. The top panel is the schematic representation of SVA-F1 elements with different numbers of BORIS binding sites depending on the length of 5â -transduced sequence. The bottom panel is the plot showing the average tag density of BORIS ChIP-Seq across the transduced sequences of different length.