Additional file 6: of Inhibition of bromodomain and extra-terminal (BET) proteins increases NKG2D ligand MICA expression and sensitivity to NK cell-mediated cytotoxicity in multiple myeloma cells: role of cMYC-IRF4-miR-125b interplay
Published in 2016 by
Maria Pia Abruzzese,
Maria Bilotta,
Cinzia Fionda,
Alessandra Zingoni,
Alessandra Soriani,
Elisabetta Vulpis,
Cristiana Borrelli,
Beatrice Zitti,
Maria Petrucci,
Maria Ricciardi,
Rosa Molfetta,
Rossella Paolini,
Angela Santoni,
Marco Cippitelli
Abstract
(A) Schematic representation of IKAROS and IRF4 response elements of MICA and MICB promoters. (B, C) Effect of BETi on IKZF1 and IKZF3 mRNA expression in SKO-007(J3) MM cells. Real-time PCR analysis of total mRNA obtained from SKO-007(J3) cells, untreated or treated with JQ1 (0.5 μM) or lenalidomide (5 μM) for 48 h. Data, expressed as fold change units, were normalized with GAPDH and referred to the untreated cells considered as calibrator and represent the mean of three experiments (*P