Figure S2. Analysis of Sox2-null ES cells rescued by Sox 17 carrying the E57K mutation. A Rescue assay of Sox17E57K in which E57 was replaced by K. The numbers of the primary colonies of transfectants with the standard deviation are indicated under the gene symbols. Column1 shows colonies of primary transfectants grown without Tc. Column 2 shows secondary colonies grown in the presence of Tc. Stem cell colonies were recognized their tightly packed morpohology. Column 3 shows colonies at passage 3 in the presence of Tc if they yielded stem cell colonies at this stage. B Immunostaining of rescued ES cells. The rescued ES cells at passage 4 in the presence of Tc were stained for Sox2, Oct3/4, Nanog and Klf4. The absence of Sox2 staining with goat anti-Sox2 polyclonal antibody in Sox17E57K transfectants confirmed the rescue. C Immunostaining of Sox2-null ES cells rescued by Sox17E57K with anti-Sox17 antibody. Ectopic expression of Sox17E57K in rescued ES cells was confirmed. 2TS22C ES cells cultured without Tc serve as a negative control (PDF 932 kb)