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American Society for Microbiology, Applied and Environmental Microbiology, 2(72), p. 1705-1707, 2006

DOI: 10.1128/aem.72.2.1705-1707.2006

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Isolation of Rickettsia felis in the Mosquito Cell Line C6/36

Journal article published in 2006 by Maurício C. Horta, Marcelo B. Labruna, Edison L. Durigon ORCID, Teresinha T. S. Schumaker
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT We report the isolation and establishment of Rickettsia felis in the C6/36 cell line. Rickettsial growth was intense, always with 90 to 100% of cells being infected after few weeks. The rickettsial isolate was confirmed by testing infected cells by PCR and sequencing fragments of three major Rickettsia genes ( gltA , ompB , and the 17-kDa protein gene).