In the context of noncommutative space-time, we investigate the nucleon structure functions which plays an important role to identify the internal structure of nucleons. We use the corrected vertices and employ new vertices that appear in two approaches of noncommutativity and calculate the proton structure functions in terms of noncommutative tensor \theta_{μν}. To check our result, we plot the nucleon structure function (NSF), F_2(x), and compare it with experimental data and the result coming out from the GRV, GJR and CT10 parametrization models. We show that new vertex which is arising the noncommutativity correction will lead us to better consistency between theoretical result and experimental data for NSF. This consistency would be better at small values of x-Bjorken variable. To indicate and confirm the validity of our calculations, we also act conversely and obtain an lower bound for the numerical values of Λ_{NC} scale which are corresponding to the recent reports. ; Comment: 28 pages, 5 figuree