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Elsevier, Data in Brief, (7), p. 714-720, 2016

DOI: 10.1016/j.dib.2016.03.025

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Data supporting the rat brain sample preparation and validation assays for simultaneous determination of 8 neurotransmitters and their metabolites using liquid chromatography-tandem mass spectrometry

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Data provided by SHERPA/RoMEO

Abstract

The data presented in this article supports the rat brain sample pre- parathion procedure previous to its injection into the liquid chroma- tography–tandem mass spectrometry(LC–MS/MS) system to monitor levels of adrenaline, noradrenaline, glutamicacid, γ-aminobutyric acid, dopamine, 5-hydroxytryptamine, 5-hydroxyindoleaceticacid, and 3-methoxy-4-hydroxyphenylglycol.In addition,we describe the method validation assays (such as calibration curve, lower limit of quantification, precision and accuracy intra-and inter-day,selectivity, extraction recovery and matrix effect, stability,and carry-over effect) according to the United States Food and Drug Administration and European Medicine Agency to measure in one step different neuro- transmitters and their metabolites.The data supplied in this articleis related to the research study entitled: “Simultaneous determination of 8 neurotransmitters and their metabolite levels in rat brainusing liquid chromatography in tandem with mass spectrometry: application to the murine Nrf2 model of depression” (Wojniczetal.2016)