AbstractThe surface brightness fluctuation (SBF) method at near-infrared (NIR) wavelengths is a powerful tool for estimating distances to unresolved stellar systems with high precision. The IR channel of the Wide Field Camera 3 (WFC3), installed on board the Hubble Space Telescope (HST) in 2009, has a greater sensitivity and a wider field of view than the previous generation of HST IR instruments, making it much more efficient for measuring distances to early-type galaxies in the Local Volume. To take full advantage of its capabilities, we need to empirically calibrate the SBF distance method for WFC3's NIR passbands. We present the SBF measurements for the WFC3/IR F160W bandpass filter using observations of 16 early-type galaxies in the Fornax and Virgo Clusters. These have been combined with existing (g₄₇₅–z₈₅₀) color measurements from the Advanced Camera for Surveys Virgo and Fornax Cluster Surveys to derive a space-based H₁₆₀-band SBF relation as a function of color. We have also compared the absolute SBF magnitudes to those predicted by evolutionary population synthesis models in order to study stellar population properties in the target galaxies.