Springer (part of Springer Nature), Analytical and Bioanalytical Chemistry, 8(407), p. 2027-2033
DOI: 10.1007/s00216-014-8357-8
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IntroductionMass spectrometry (MS) imaging is a very active field of research, and has seen impressive progress in recent years [1, 2]. The number of groups that are working on this topic is constantly increasing. However, the field is still very heterogeneous in terms of applied instrumentation and data processing methods. In addition, complex datasets are reduced to a set of two-dimensional “images,” which inevitably results in information loss. This simplified graphical representation also strongly depends on processing options such as color scale, intensity normalization, and spatial interpolation. Consequently, experimental data are presented in very diverse ways, and published results can therefore be difficult to evaluate and compare. With a growing number of published studies, the issue of standardization and quality control of MS imaging data is becoming more important. This is a natural process for any new field that is maturing. The MS-based proteomics community has been fac ...