The bone substation grafts, such as hydroxyapatite, TCP, have been used in the clinical. But it is found that they are not capable of enhancing bone regeneration due to their poor osteoinductive compared with allograft and autograft. In order to increase the osteoinductive, bone morphogenetic protein 2 (BMP-2) has highlighted to incorporate into a three dimensional reservoir. The purpose of the present study was to develop a novel drug delivery system with the capability of controlled releasing BMP-2. In this study, BMP-2/Chitosan (CS) microspheres were succeeded loaded on the deproteinized bovine bone (DBB) scaffold. The release profile of BMP-2 study indicated an initial burst release followed by a sustained release. In vitro bioactivity assay revealed that the encapsulated growth factor was biologically active as evidenced by cell culture. Therefore, the novel strategy of this microspheres-scaffold system is potentially to utilize the bone tissue engineered.