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American Society for Microbiology, Journal of Clinical Microbiology, 5(50), p. 1718-1720, 2012

DOI: 10.1128/jcm.00195-12

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Ultrarapid Detection of blaKPC1/2-12 from Perirectal and Nasal Swabs by Use of Real-Time PCR

Journal article published in 2012 by Sara N. Richter ORCID, Ilaria Frasson, Maria Angela Biasolo, Andrea Bartolini, Antonietta Cavallaro, Giorgio Palù
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT The novel real-time PCR assay developed as described here was able to detect bla KPC1/2-12 ( bla KPC-1/2 to bla KPC-12 ) from easily available clinical specimens in less than 2 h. The genotypic assay was highly sensitive (100%) and specific (98%). In some cases, it was able to detect bla KPC 48 h before positive detection by standard phenotypic assay on patients who were monitored daily. The high sensitivity and rapidity of the molecular method make it the method of choice for KPC surveillance and, thus, containment purposes.