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Nature Research, Nature Methods, 3(12), p. 230-232, 2014

DOI: 10.1038/nmeth.3152

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Coverage recommendations for methylation analysis by whole genome bisulfite sequencing

Journal article published in 2014 by Michael J. Ziller, Kasper Daniel Hansen ORCID, Alexander Meissner, Martin J. Aryee ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Whole genome bisulfite sequencing (WGBS) allows genome-wide DNA methylation profiling but the associated high sequencing costs continue to limit its widespread application. We utilized several high coverage reference data sets to experimentally determine minimal sequencing requirements. Here, we present data derived recommendations for minimum sequencing depth for WGBS libraries, highlight what is gained with increasing coverage and discuss the trade off between sequencing depth and number of assayed replicates.