Dissemin is shutting down on January 1st, 2025

Published in

Cell Press, Molecular Therapy, 12(18), p. 2085-2093, 2010

DOI: 10.1038/mt.2010.189

Links

Tools

Export citation

Search in Google Scholar

The Effect of Cell Cycle Synchronization on Tumor Sensitivity to Reovirus Oncolysis

Journal article published in 2010 by Lucy Heinemann, Guy R. Simpson, Nicola E. Annels, Richard Vile, Alan Melcher ORCID, Robin Prestwich, Kevin J. Harrington ORCID, Hardev S. Pandha
This paper is available in a repository.
This paper is available in a repository.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Orange circle
Postprint: archiving restricted
Upload
Red circle
Published version: archiving forbidden
Policy details
Data provided by SHERPA/RoMEO

Abstract

The potential for increased sensitivity of tumor cells to oncolytic reovirus by altering the normal cell cycle using clinically available pharmacological agents was investigated. B16.F10 mouse melanoma cells were partially synchronized with hydroxyurea, thymidine, or by mitotic shake-off. Cell survival was determined using MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)- 2-(4-sulfophenyl)-2H-tetrazolium)] survival assay and virus yield in tumors by plaque assay. An enhanced sensitivity to reovirus was observed following the removal of either hydroxyurea or thymidine from the culture medium (P