Published in

American Society for Microbiology, Applied and Environmental Microbiology, 8(76), p. 2574-2581, 2010

DOI: 10.1128/aem.01992-09

Links

Tools

Export citation

Search in Google Scholar

Metabolomic Characterization of the Salt Stress Response in Streptomyces coelicolor

Journal article published in 2010 by Stefan Kol, Marcel de Vries, M. Elena Merlo, M. Elena Merlo, Richard A. Scheltema, M. De Vries, Roel J. Vonk, Niels A. Kikkert, Lubbert Dijkhuizen, Rainer Breitling ORCID, Eriko Takano
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Green circle
Postprint: archiving allowed
Upload
Orange circle
Published version: archiving restricted
Upload
Policy details
Data provided by SHERPA/RoMEO

Abstract

The humicolous actinomycete Streptomyces coelicolor routinely adapts to a wide variety of habitats and rapidly changing environments. Upon salt stress, the organism is also known to increase the levels of various compatible solutes. Here we report the results of the first high-resolution metabolomics time series analysis of various strains of S. coelicolor exposed to salt stress: the wild type, mutants with progressive knockouts of the ectoine biosynthesis pathway, and two stress regulator mutants (with disruptions of the sigB and osaB genes). Samples were taken from cultures at 0, 4, 8, and 24 h after salt stress treatment and analyzed by liquid chromatography-mass spectrometry with an LTQ Orbitrap XL mass spectrometer. The results suggest that a large fraction of amino acids is upregulated in response to the salt stress, as are proline/glycine-containing diand tripeptides. Additionally we found that 5'-methylthioadenosine, a known inhibitor of polyamine biosynthesis, is downregulated upon salt stress. Strikingly, no major differences between the wild-type cultures and the two stress regulator mutants were found, indicating a considerable robustness of the metabolomic response to salt stress, compared to the more volatile changes in transcript abundance reported earlier. Copyright ?? 2010, American Society for Microbiology. All Rights Reserved.