Published in

National Academy of Sciences, Proceedings of the National Academy of Sciences, 42(106), p. 17741-17746, 2009

DOI: 10.1073/pnas.0905177106



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Kinesin's step dissected with single-motor FRET

Journal article published in 2009 by Sander Verbrugge, Zdenek Lansky, Erwin J. G. Peterman ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Data provided by SHERPA/RoMEO


The motor protein Kinesin-1 drives intracellular transport along microtubules, with each of its two motor domains taking 16-nm steps in a hand-over-hand fashion. The way in which a single-motor domain moves during a step is unknown. Here, we use Förster resonance energy transfer (FRET) between fluorescent labels on both motor domains of a single kinesin. This approach allows us to resolve the relative distance between the motor domains and their relative orientation, on the submillisecond timescale, during processive stepping. We observe transitions between high and low FRET values for certain kinesin constructs, depending on the location of the labels. These results reveal that, during a step, a kinesin motor domain dwells in a well-defined intermediate position for approximately 3 ms.