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Oxford University Press (OUP), Bioinformatics, 4(30), p. 566-568

DOI: 10.1093/bioinformatics/btt702

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NextClip: an analysis and read preparation tool for Nextera Long Mate Pair libraries

Journal article published in 2013 by Richard M. Leggett, Bernardo J. Clavijo ORCID, Leah Clissold, Matthew D. Clark, Mario Caccamo
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Summary: Illumina’s recently released Nextera Long Mate Pair (LMP) kit enables production of jumping libraries of up to 12 kb. The LMP libraries are an invaluable resource for carrying out complex assemblies and other downstream bioinformatics analyses such as the characterization of structural variants. However, LMP libraries are intrinsically noisy and to maximize their value, post-sequencing data analysis is required. Standardizing laboratory protocols and the selection of sequenced reads for downstream analysis are non-trivial tasks. NextClip is a tool for analyzing reads from LMP libraries, generating a comprehensive quality report and extracting good quality trimmed and deduplicated reads.