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American Chemical Society, Nano Letters, 10(5), p. 1883-1888, 2005

DOI: 10.1021/nl0510816

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Stretching DNA using the Electric Field in a Synthetic Nanopore

Journal article published in 2005 by Jiunn B. Heng, Aleksei Aksimentiev ORCID, Chuen Ho, Patrick Marks, Yelena V. Grinkova, Steve Sligar, Klaus Schulten, Gregory Timp
This paper is available in a repository.
This paper is available in a repository.

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Abstract

The mechanical properties of DNA over segments comparable to the size of a protein-binding site (3-10 nm) are examined using an electric-field-induced translocation of single molecules through a nanometer diameter pore. DNA, immersed in an electrolyte, is forced through synthetic pores ranging from 0.5 to 1.5 nm in radius in a 10 nm thick Si(3)N(4) membrane using an electric field. To account for the stretching and bending, we use molecular dynamics to simulate the translocation. We have found a threshold for translocation that depends on both the dimensions of the pore and the applied transmembrane bias. The voltage threshold coincides with the stretching transition that occurs in double-stranded DNA near 60 pN.