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American Chemical Society, Nano Letters, 6(7), p. 1680-1685, 2007

DOI: 10.1021/nl070668c

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Detecting SNPs using a Synthetic Nanopore

Journal article published in 2007 by Q. Zhao, G. Sigalov, V. Dimitrov, B. Dorvel, U. Mirsaidov ORCID, S. Sligar, and A. Aksimentiev ORCID, G. Timp
This paper is available in a repository.
This paper is available in a repository.

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Abstract

We have discovered a voltage threshold for permeation through a synthetic nanopore of dsDNA bound to a restriction enzyme that depends on the sequence. Molecular dynamic simulations reveal that the threshold is associated with a nanonewton force required to rupture the DNA-protein complex. A single mutation in the recognition site for the restriction enzyme, i.e., a single nucleotide polymorphism (SNP), can easily be detected as a change in the threshold voltage. Consequently, by measuring the threshold voltage in a synthetic nanopore, it may be possible to discriminate between two variants of the same gene (alleles) that differ in one base.