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F1000Research, F1000Research, (5), p. 317, 2016

DOI: 10.12688/f1000research.8170.1

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Autocrine and paracrine Wingless signalling in the Drosophila midgut by both continuous gradient and asynchronous bursts of wingless expression

Journal article published in 2016 by Hsiao Yu Fang, Alfonso Martinez-Arias, Joaquín de Navascués
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Wingless (Wg)/ Wnt signalling is a major regulator of homeostasis in both the mammalian andDrosophilaintestine. InDrosophilathe organisation and function of Wingless signalling in the adult intestine remain poorly understood. Here we characterise the pattern of expression ofwg, the stabilisation of its effector Armadillo in the adultDrosophilamidgut, and correlate them with the response of the cells to Wg signalling activation. We show that in normal homeostasis there is a gradient of Wingless signalling in the intestinal stem cell (ISC) and the undifferentiated progenitor cell (enteroblast, EB) populations along the posterior midgut, with a high point at the midgut-hindgut boundary (pylorus). This gradient results from a combination of two sources of Wingless: a distant source outside the epithelium (the pylorus) and a local one from the ISCs and EBs themselves. Altogether, our studies show that Wingless expression and signalling in the epithelium is not continuous, but operates through bursts that occur randomly in space and time.