Figure S2. Effect of vehicle or SA repeated treatment (2 mg/kg, i.p.), alone or in presence of nor-BNI (20 mg/kg, i.p.), or AM251 (1 mg/kg, i.p.) on spinal astrocytes in mice receiving formalin into the hind-paws. GFAP immunoreactivity (GFAP-ir) is shown in the ipsilateral dorsal horn 7 days after formalin (A-D). Quantitative analysis of percentage of activated astrocytes on the total cell number in L4-L6 spinal cord sections is shown in “E”. Data are expressed as mean ± S.E.M of 3 mice per group. * and ○ indicate statistically significant differences vs veh/form and SA/form, respectively. (P <0.05, one-way ANOVA, Tukey post hoc). (PPT 303 kb)