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Wiley, The Plant Journal, 5(85), p. 660-674, 2016

DOI: 10.1111/tpj.13133

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Proteomic analysis reveals the differential histone programmes between male germline cells and vegetative cells in Lilium davidii

Journal article published in 2016 by Hao Yang, Ning Yang, Tai Wang
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

In flowering plants, male germline fate is determined after asymmetric division of the haploid microspore. Daughter cells have distinct fates: the generative cell (GC) undergoes further mitosis to generate sperm cells (SCs), and the vegetative cell (VC) terminally differentiates. However, our understanding of the mechanisms underlying germline development remains limited. Histone variants and modifications define chromatin states and contribute to establishing and maintaining cell identities by affecting gene expression. Here, we constructed a lily protein database, then extracted and detailed histone entries into a comprehensive lily histone database. We isolated a large amount of nuclei from VCs, GCs and SCs from lily. We profiled histone variants of all five histone families in all three cell types by proteomics approaches. We revealed 92 identities representing 32 histone variants: 6 for H1, 11 for H2A, 8 for H2B, 5 for H3 and 2 for H4 families. Nine, including 5 H1, 2 H2B, 1 H3 and 1 H4 variants, were specifically accumulated in GCs and SCs. We also detected H3 modification patterns in the three cell types. GCs and SCs had almost identical histone profiles and similar H3 modification patterns, which were significantly different from those of VCs. Our study also revealed multiple isoforms and differential expression patterns between isoforms of a variant. The results suggest that differential histone programmes between the germline and companion VCs may be established following the asymmetric division, and are important for identity establishment and differentiation of the male germline as well as the VC. This article is protected by copyright. All rights reserved.